Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, ROS1728 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
66482-1-Ig targets EIF2A/CDA02 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | EIF2A/CDA02 fusion protein Ag22041 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 2A, 65kDa |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | BC011885 |
| Gene Symbol | EIF2A |
| Gene ID (NCBI) | 83939 |
| RRID | AB_2881848 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BY44 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
| IHC protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
| IF protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Pharm Biol Cinobufagin induces acute promyelocytic leukaemia cell apoptosis and PML-RARA degradation in a caspase-dependent manner by inhibiting the β-catenin signalling pathway | ||
Front Neurosci Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease |