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EIF2A/CDA02 Monoclonal antibody
EIF2A/CDA02 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1G7H4
Cat no : 66482-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, ROS1728 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
Product Information
66482-1-Ig targets EIF2A/CDA02 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | EIF2A/CDA02 fusion protein Ag22041 相同性解析による交差性が予測される生物種 |
Full Name | eukaryotic translation initiation factor 2A, 65kDa |
Calculated molecular weight | 65 kDa |
Observed molecular weight | 65 kDa |
GenBank accession number | BC011885 |
Gene symbol | EIF2A |
Gene ID (NCBI) | 83939 |
RRID | AB_2881848 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.
Protocols
Product Specific Protocols | |
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WB protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
IHC protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
IF protocol for EIF2A/CDA02 antibody 66482-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Pharm Biol Cinobufagin induces acute promyelocytic leukaemia cell apoptosis and PML-RARA degradation in a caspase-dependent manner by inhibiting the β-catenin signalling pathway | ||
Front Neurosci Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Dorota (Verified Customer) (04-10-2019) | Very good for western blotting.
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