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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EIF2A/CDA02 Monoclonal antibody (66482-1-Ig)

    WB analysis of HEK-293 using 66482-1-Ig (same clone as 66482-1-PBS)

    WB result of EIF2A/CDA02 antibody (66482-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2A/CDA02 transfected HEK-293 cells. This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EIF2A/CDA02 Monoclonal antibody (66482-1-Ig)

    WB analysis using 66482-1-Ig (same clone as 66482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66482-1-Ig (EIF2A/CDA02 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using EIF2A/CDA02 Monoclonal antibody (66482-1-Ig)

    WB analysis of ROS1728 using 66482-1-Ig (same clone as 66482-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66482-1-Ig (EIF2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EIF2A/CDA02 Monoclonal antibody (66482-1-Ig)

    IHC staining of human prostate cancer using 66482-1-Ig (same clone as 66482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66482-1-Ig (EIF2A antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EIF2A/CDA02 Monoclonal antibody (66482-1-Ig)

    IHC staining of human prostate cancer using 66482-1-Ig (same clone as 66482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66482-1-Ig (EIF2A antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF2A/CDA02 Monoclonal antibody (66482-1-Ig)

    IF Staining of HepG2 using 66482-1-Ig (same clone as 66482-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using EIF2A/CDA02 antibody (66482-1-Ig, Clone: 1G7H4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

EIF2A/CDA02 Monoclonal antibody, PBS Only

EIF2A/CDA02 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66482-1-PBS
Clone No.1G7H4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EIF2A, EIF2, EIF 2A, EIF, CDA02

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66482-1-PBS targets EIF2A/CDA02 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF2A/CDA02 fusion protein Ag22041 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2A, 65kDa
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011885
Gene Symbol EIF2A
Gene ID (NCBI) 83939
RRIDAB_2881848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BY44
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.