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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

EIF2AK1 Recombinant antibody, PBS Only

EIF2AK1 Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

3J9

Cat no : 81768-1-PBS

Synonyms

Heme regulated inhibitor, Heme controlled repressor, HCR, Eukaryotic translation initiation factor 2-alpha kinase 1, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EIF2AK1 Recombinant antibody (81768-1-RR)

    WB analysis of HEK-293 using 81768-1-RR (same clone as 81768-1-PBS)

    WB result of EIF2AK1 antibody (81768-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 81768-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EIF2AK1 Recombinant antibody (81768-1-RR)

    WB analysis of HEK-293 using 81768-1-RR (same clone as 81768-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 81768-1-RR (EIF2AK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81768-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81768-1-RR

    Affinity and Kinetic Characterization of 81768-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81768-1-RR against Human EIF2AK1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81768-1-PBS targets EIF2AK1 in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EIF2AK1 fusion protein Ag14236 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2-alpha kinase 1
Calculated molecular weight 630 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC006524
Gene symbol EIF2AK1
Gene ID (NCBI) 27102
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.