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  • KD/KO Validated

EIF2AK1 Recombinant antibody

EIF2AK1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3J9

Cat no : 81768-1-RR

Synonyms

Heme regulated inhibitor, Heme controlled repressor, HCR, Eukaryotic translation initiation factor 2-alpha kinase 1, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EIF2AK1 Recombinant antibody (81768-1-RR)

    WB analysis of HEK-293 using 81768-1-RR

    WB result of EIF2AK1 antibody (81768-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using EIF2AK1 Recombinant antibody (81768-1-RR)

    WB analysis of HEK-293 using 81768-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 81768-1-RR (EIF2AK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 81768-1-RR

    Affinity and Kinetic Characterization of 81768-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81768-1-RR against Human EIF2AK1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81768-1-RR targets EIF2AK1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EIF2AK1 fusion protein Ag14236 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2-alpha kinase 1
Calculated molecular weight 630 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC006524
Gene symbol EIF2AK1
Gene ID (NCBI) 27102
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

Protocols

Product Specific Protocols
WB protocol for EIF2AK1 antibody 81768-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols