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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated EIF2AK2, PKR Polyclo (CL488-18244)

    IF Staining of HeLa using CL488-18244

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 EIF2AK2, PKR antibody (CL488-18244) at dilution of 1:200.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated EIF2AK2, PKR Polyclonal antibody

EIF2AK2, PKR Polyclonal Antibody for IF/ICC
Cat No. CL488-18244

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

IF/ICC

Synonyms

eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18244 targets EIF2AK2, PKR in IF/ICC applications and shows reactivity with human, mouse , rat samples.

Tested Reactivity human, mouse , rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2AK2, PKR fusion protein Ag13010 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2-alpha kinase 2
Calculated molecular weight 551 aa, 62 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC093676
Gene Symbol EIF2AK2
Gene ID (NCBI) 5610
RRIDAB_3084018
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19525
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EIF2AK2, PKR antibody CL488-18244Download protocol
Standard Protocols
Click here to view our Standard Protocols