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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using EIF2B1 Polyclonal antibody (18010-1-AP)

    WB analysis of MCF-7 using 18010-1-AP (same clone as 18010-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using EIF2B1 Polyclonal antibody (18010-1-AP)

    WB analysis of K-562 using 18010-1-AP (same clone as 18010-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF2B1 Polyclonal antibody (18010-1-AP)

    WB analysis of HeLa using 18010-1-AP (same clone as 18010-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using EIF2B1 Polyclonal antibody (18010-1-AP)

    IP experiment of K-562 using 18010-1-AP (same clone as 18010-1-PBS)

    IP result of anti-EIF2B1 (IP:18010-1-AP, 4ug; Detection:18010-1-AP 1:700) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of human breast cancer using 18010-1-AP (same clone as 18010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of human breast cancer using 18010-1-AP (same clone as 18010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of mouse brain using 18010-1-AP (same clone as 18010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF2B1 Polyclonal antibody (18010-1-AP)

    IF Staining of HeLa using 18010-1-AP (same clone as 18010-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2B1 antibody (18010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18010-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

EIF2B1 Polyclonal antibody, PBS Only

EIF2B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18010-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18010-1-PBS targets EIF2B1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2B1 fusion protein Ag12509 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
Calculated molecular weight 305 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC103763
Gene Symbol EIF2B1
Gene ID (NCBI) 1967
RRIDAB_2261996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14232
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.