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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated EIF3F Recombinant an (CL488-84131-2)

    IF Staining of HeLa using CL488-84131-2

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 EIF3F antibody (CL488-84131-2, Clone: 240894D2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated EIF3F Recombinant an (CL488-84131-2)

    FC experiment of U2OS using CL488-84131-2

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Eif3f Recombinant Antibody (CL488-84131-2, Clone:240894D2)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated EIF3F Recombinant antibody

EIF3F Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-84131-2
Clone No.240894D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

eIF3 p47, 240894D2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84131-2 targets EIF3F in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EIF3F fusion protein Ag34849 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit F
Calculated molecular weight 38 kDa
Observed molecular weight40-45 kDa
GenBank accession numberBC000490
Gene Symbol EIF3F
Gene ID (NCBI) 8665
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00303
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF3F (eukaryotic translation initiation factor 3 subunit F) is a protein that plays an important role in eukaryotic cells and is an integral part of the translation initiation factor 3(Eif3) complex. EIF3 is a complex of at least 10 distinct subunits and is the largest translation initiation factor in eukaryotes.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EIF3F antibody CL488-84131-2Download protocol
Standard Protocols
Click here to view our Standard Protocols