CoraLite®594-conjugated EIF3M Monoclonal antibody
EIF3M Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2A12B2
Cat no : CL594-66025
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-66025 targets EIF3M in IF/ICC applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | EIF3M fusion protein Ag17965 相同性解析による交差性が予測される生物種 |
Full Name | eukaryotic translation initiation factor 3, subunit M |
Calculated molecular weight | 374 aa, 43 kDa |
Observed molecular weight | 43 kDa |
GenBank accession number | BC019103 |
Gene symbol | EIF3M |
Gene ID (NCBI) | 10480 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 EIF3M antibody CL594-66025 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |