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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF4A2 Monoclonal antibody (67799-1-Ig)

    WB analysis using 67799-1-Ig (same clone as 67799-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67799-1-Ig (EIF4A2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using EIF4A2 Monoclonal antibody (67799-1-Ig)

    IF Staining of HEK-293 using 67799-1-Ig (same clone as 67799-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using EIF4A2 Monoclonal antibody (67799-1-Ig)

    IF Staining of HEK-293 using 67799-1-Ig (same clone as 67799-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

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EIF4A2 Monoclonal antibody, PBS Only

EIF4A2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67799-1-PBS
Clone No.2D11E11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EIF4A, eIF 4A II, EC:3.6.4.13, DDX2B, BM 010

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67799-1-PBS targets EIF4A2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EIF4A2 fusion protein Ag9085 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4A, isoform 2
Calculated molecular weight 408 aa, 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC012547
Gene Symbol EIF4A2
Gene ID (NCBI) 1974
RRIDAB_2918563
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14240
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.