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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF4A2 Monoclonal antibody (67799-1-Ig)

    WB analysis using 67799-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67799-1-Ig (EIF4A2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using EIF4A2 Monoclonal antibody (67799-1-Ig)

    IF Staining of HEK-293 using 67799-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using EIF4A2 Monoclonal antibody (67799-1-Ig)

    IF Staining of HEK-293 using 67799-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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EIF4A2 Monoclonal antibody

EIF4A2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67799-1-Ig
Clone No.2D11E11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EIF4A, eIF 4A II, EC:3.6.4.13, DDX2B, BM 010

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67799-1-Ig targets EIF4A2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EIF4A2 fusion protein Ag9085 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4A, isoform 2
Calculated molecular weight 408 aa, 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC012547
Gene Symbol EIF4A2
Gene ID (NCBI) 1974
RRIDAB_2918563
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14240
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

Protocols

Product Specific Protocols
WB protocol for EIF4A2 antibody 67799-1-IgDownload protocol
IF protocol for EIF4A2 antibody 67799-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols