CoraLite® Plus 488-conjugated EIF4A2 Monoclonal antibody
EIF4A2 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Rat, Mouse
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D11E11
Cat no : CL488-67799
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
| Positive FC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67799 targets EIF4A2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Rat, Mouse samples.
| Tested Reactivity | Human, Rat, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | EIF4A2 fusion protein Ag9085 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 4A, isoform 2 |
| Calculated molecular weight | 408 aa, 46 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC012547 |
| Gene symbol | EIF4A2 |
| Gene ID (NCBI) | 1974 |
| RRID | AB_2919551 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 EIF4A2 antibody CL488-67799 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
