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CoraLite® Plus 647-conjugated EIF4A2 Monoclonal antibody

EIF4A2 Monoclonal Antibody for FC (Intra)
Cat No. CL647-67799
Clone No.2D11E11

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive FC (Intra) detected inHEK-293 cells, HeLa cells
Positive FC detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67799 targets EIF4A2 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EIF4A2 fusion protein Ag9085 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4A, isoform 2
Calculated molecular weight 408 aa, 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC012547
Gene Symbol EIF4A2
Gene ID (NCBI) 1974
RRIDAB_2935087
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14240
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 EIF4A2 antibody CL647-67799Download protocol
Standard Protocols
Click here to view our Standard Protocols
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