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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated EIF4E Monoclonal antibody (CL594-66655)

    IF Staining of HepG2 using CL594-66655

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 EIF4E antibody (CL594-66655, Clone: 3C6B9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated EIF4E Monoclonal antibody (CL594-66655)

    FC experiment of HepG2 using CL594-66655

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EIF4E (CL594-66655, Clone:3C6B9) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite®594-conjugated EIF4E Monoclonal antibody

EIF4E Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66655
Clone No.3C6B9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

eIF-4F 25 kDa subunit, EIF4F, EIF4EL1, eIF-4E, eIF 4E

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66655 targets EIF4E in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF4E fusion protein Ag27191 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4E
Calculated molecular weight 29 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC012611
Gene Symbol EIF4E
Gene ID (NCBI) 1977
RRIDAB_2883611
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06730
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic translation initiation factor 4E(EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap-binding component of the translation initiation complex eIF4F. The other subunits of EIF4F are a 50-kD polopeptied, terned EIF4A and a 220-kD polypeptied EIF4G. EIF4E mediates the binding to the mRNA cap then repress the translation. Due to the increasing expression of c-Myc in the oncogene, EIF4E's activity is enhance.

Protocols

Product Specific Protocols
IF protocol for CL594 EIF4E antibody CL594-66655Download protocol
FC protocol for CL594 EIF4E antibody CL594-66655Download protocol
Standard Protocols
Click here to view our Standard Protocols