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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF5A Monoclonal antibody

EIF5A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D1B6

Cat no : 67214-1-Ig

Synonyms

eIF-4D, eIF 5A1, eIF 5A 1, EIF 5A, eIF 4D

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF5A Monoclonal antibody (67214-1-Ig)

    WB analysis using 67214-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF5A Monoclonal antibody (67214-1-Ig)

    WB analysis using 67214-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF5A Monoclonal antibody (67214-1-Ig)

    WB analysis using 67214-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF5A Monoclonal antibody (67214-1-Ig)

    WB analysis using 67214-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using EIF5A Monoclonal antibody (67214-1-Ig)

    IF Staining of HEK-293 using 67214-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF5A antibody (67214-1-Ig, Clone: 2D1B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, LNCaP cells, Jurkat cells, NIH/3T3 cells, HEK-293 cells, PC-3 cells, HSC-T6 cells, RAW 264.7 cells, HaCaT cells, HepG2 cells, SK-BR-3 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67214-1-Ig targets EIF5A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF5A fusion protein Ag28588 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 5A
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC001832
Gene symbol EIF5A
Gene ID (NCBI) 1984
RRIDAB_2882506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.

Protocols

Product Specific Protocols
WB protocol for EIF5A antibody 67214-1-IgDownload protocol
IF protocol for EIF5A antibody 67214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols