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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF5A Monoclonal antibody, PBS Only

EIF5A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D1B6

Cat no : 67214-1-PBS

Synonyms

eIF-4D, eIF 5A1, eIF 5A 1, EIF 5A, eIF 4D

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EIF5A Monoclonal antibody (67214-1-Ig)

    WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EIF5A Monoclonal antibody (67214-1-Ig)

    WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EIF5A Monoclonal antibody (67214-1-Ig)

    WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EIF5A Monoclonal antibody (67214-1-Ig)

    WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using EIF5A Monoclonal antibody (67214-1-Ig)

    IF Staining of HEK-293 using 67214-1-Ig (same clone as 67214-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF5A antibody (67214-1-Ig, Clone: 2D1B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67214-1-PBS targets EIF5A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF5A fusion protein Ag28588 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 5A
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC001832
Gene symbol EIF5A
Gene ID (NCBI) 1984
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.