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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated EIF5A2 Monoclonal an (CL488-67907)

    IF Staining of HepG2 using CL488-67907

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 EIF5A2 antibody (CL488-67907, Clone: 1F9B2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated EIF5A2 Monoclonal an (CL488-67907)

    FC experiment of HepG2 using CL488-67907

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EIF5A2 (CL488-67907, Clone:1F9B2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 488-conjugated EIF5A2 Monoclonal antibody

EIF5A2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67907
Clone No.1F9B2

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Synonyms

EIF5A1/EIF5A2, eIF5AII, eIF-5A-2, eIF-5A2, EIF 5A2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67907 targets EIF5A2 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF5A2 fusion protein Ag10852 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 5A2
Calculated molecular weight 153 aa, 17 kDa
Observed molecular weight20 kDa
GenBank accession numberBC036072
Gene Symbol EIF5A2
Gene ID (NCBI) 56648
RRIDAB_2934587
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9GZV4
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic translation initiation factor 5A (EIF5A), a conserved lysine to hypusine, is strictly indispensable for the survival of eukaryotic cells. There are two EIF5A isoforms in humans: EIF5A1 and EIF5A2. EIF5A2 has been reported to be overexpressed in gallbladder cancer, upper urinary tract urothelial carcinoma, gastric cancer, prostate cancer, nasopharyngeal carcinoma, and cervical cancer. EIF5A2 upregulation in cancer tissues is associated with poor prognosis in these patients.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EIF5A2 antibody CL488-67907Download protocol
FC protocol for CL Plus 488 EIF5A2 antibody CL488-67907Download protocol
Standard Protocols
Click here to view our Standard Protocols