Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, PC-12 cells, Neuro-2a cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
67097-1-Ig targets ELAVL2 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ELAVL2 fusion protein Ag28310 相同性解析による交差性が予測される生物種 |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B) |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC030692 |
| Gene Symbol | ELAVL2 |
| Gene ID (NCBI) | 1993 |
| RRID | AB_2882402 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q12926 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ELAVL2 antibody 67097-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis. | ||
Sci Adv The long noncoding RNA mimi scaffolds neuronal granules to maintain nervous system maturity | ||
Mol Neurodegener Increased expression of mesencephalic astrocyte-derived neurotrophic factor (MANF) contributes to synapse loss in Alzheimer's disease |