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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ELAVL2 Monoclonal antibody, PBS Only (Capture)

ELAVL2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D8C10

Cat no : 67097-1-PBS

Synonyms

Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2, ELAV2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis using 67097-1-Ig (same clone as 67097-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of Neuro-2a using 67097-1-Ig (same clone as 67097-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of Neuro-2a using 67097-1-Ig (same clone as 67097-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of pig brain using 67097-1-Ig (same clone as 67097-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of HEK-293 using 67097-1-Ig (same clone as 67097-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis using 67097-1-Ig (same clone as 67097-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of PC-12 using 67097-1-Ig (same clone as 67097-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of HEK-293 using 67097-1-Ig (same clone as 67097-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of SH-SY5Y using 67097-1-Ig (same clone as 67097-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of PC-12 using 67097-1-Ig (same clone as 67097-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50972-1

    Cytometric bead array standard curve of MP50972-1

    Cytometric bead array standard curve of MP50972-1, ELAVL2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67097-1-PBS. Detection antibody: 67097-2-PBS. Standard:Ag28310. Range: 0.391-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67097-1-PBS targets ELAVL2 as part of a matched antibody pair:

MP50972-1: 67097-1-PBS capture and 67097-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ELAVL2 fusion protein Ag28310 相同性解析による交差性が予測される生物種
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
Calculated molecular weight 39 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC030692
Gene symbol ELAVL2
Gene ID (NCBI) 1993
RRIDAB_2882402
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].