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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ELAVL2 Monoclonal antibody, PBS Only

ELAVL2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D8C10

Cat no : 67097-1-PBS

Synonyms

Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2, ELAV2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis using 67097-1-Ig (same clone as 67097-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of Neuro-2a using 67097-1-Ig (same clone as 67097-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of Neuro-2a using 67097-1-Ig (same clone as 67097-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of pig brain using 67097-1-Ig (same clone as 67097-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of HEK-293 using 67097-1-Ig (same clone as 67097-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis using 67097-1-Ig (same clone as 67097-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of PC-12 using 67097-1-Ig (same clone as 67097-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of HEK-293 using 67097-1-Ig (same clone as 67097-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of SH-SY5Y using 67097-1-Ig (same clone as 67097-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using ELAVL2 Monoclonal antibody (67097-1-Ig)

    WB analysis of PC-12 using 67097-1-Ig (same clone as 67097-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67097-1-PBS targets ELAVL2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ELAVL2 fusion protein Ag28310 相同性解析による交差性が予測される生物種
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
Calculated molecular weight 39 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC030692
Gene symbol ELAVL2
Gene ID (NCBI) 1993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].