Validation Data Gallery
Tested Applications
| Positive WB detected in | human colon tissue, human rectum tissue |
| Positive IHC detected in | human ovary tumor tissue, human lung cancer tissue, human kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human ovary tumor tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
60047-1-Ig targets EMILIN1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | EMILIN1 fusion protein Ag1016 相同性解析による交差性が予測される生物種 |
| Full Name | elastin microfibril interfacer 1 |
| Calculated molecular weight | 107 kDa |
| Observed molecular weight | 120-130 kDa |
| GenBank accession number | BC007530 |
| Gene Symbol | EMILIN1 |
| Gene ID (NCBI) | 11117 |
| RRID | AB_2100191 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y6C2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EMILIN1 antibody 60047-1-Ig | Download protocol |
| IHC protocol for EMILIN1 antibody 60047-1-Ig | Download protocol |
| IF protocol for EMILIN1 antibody 60047-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Connect Tissue Res EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro. | ||