CoraLite® Plus 488-conjugated EMILIN1 Monoclonal antibody
EMILIN1 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A5C8
Cat no : CL488-60047
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human ovary tumor tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-60047 targets EMILIN1 in IF-P applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | EMILIN1 fusion protein Ag1016 相同性解析による交差性が予測される生物種 |
| Full Name | elastin microfibril interfacer 1 |
| Calculated molecular weight | 107 kDa |
| Observed molecular weight | 120-130 kDa |
| GenBank accession number | BC007530 |
| Gene symbol | EMILIN1 |
| Gene ID (NCBI) | 11117 |
| RRID | AB_2919227 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 EMILIN1 antibody CL488-60047 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
