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Cardinal Red™-conjugated ENO1 Polyclonal antibody
ENO1 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
Cardinal Red™ Fluorescent Dye
Cat no : CR-11204
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CR-11204 targets ENO1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ENO1 fusion protein Ag1692 相同性解析による交差性が予測される生物種 |
Full Name | enolase 1, (alpha) |
Calculated molecular weight | 47 kDa |
Observed molecular weight | 47 kDa |
GenBank accession number | BC015641 |
Gene symbol | ENO1 |
Gene ID (NCBI) | 2023 |
Conjugate | Cardinal Red™ Fluorescent Dye |
Excitation/Emission maxima wavelengths | 592 nm / 611 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation.(19756395)
Protocols
Product Specific Protocols | |
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FC protocol for Cardinal Red™ ENO1 antibody CR-11204 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |