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  • KD/KO Validated

ENOX2 Polyclonal antibody

ENOX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10423-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Protein disulfide-thiol oxidoreductase, Hydroquinone [NADH] oxidase, Ecto-NOX disulfide-thiol exchanger 2, COVA1, APK1 antigen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, mouse ovary tissue, PC-3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10423-1-AP targets ENOX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENOX2 fusion protein Ag0674 相同性解析による交差性が予測される生物種
Full Name ecto-NOX disulfide-thiol exchanger 2
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC019254
Gene Symbol ENOX2
Gene ID (NCBI) 10495
RRIDAB_2099483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16206
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tumor-associated NADH oxidase (ENOX2) as a member of a family of growth-related NADH (or hydroquinone) oxidases. It functions as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock

Protocols

Product Specific Protocols
WB protocol for ENOX2 antibody 10423-1-APDownload protocol
IHC protocol for ENOX2 antibody 10423-1-APDownload protocol
IF protocol for ENOX2 antibody 10423-1-APDownload protocol
IP protocol for ENOX2 antibody 10423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Chem

Synthesis and characterization of 4,11-diaminoanthra[2,3-b]furan-5,10-diones: tumor cell apoptosis through tNOX-modulated NAD+/NADH ratio and SIRT1.

Authors - Alexander S Tikhomirov
humanIP

Am J Cancer Res

Capsaicin acts through tNOX (ENOX2) to induce autophagic apoptosis in p53-mutated HSC-3 cells but autophagy in p53-functional SAS oral cancer cells.

Authors - Chin-Fang Chang
human,mouseIP

Am J Cancer Res

Capsaicin exerts therapeutic effects by targeting tNOX-SIRT1 axis and augmenting ROS-dependent autophagy in melanoma cancer cells.

Authors - Atikul Islam
humanWB

Exp Cell Res

Phosphorylation of serine-504 of tNOX (ENOX2) modulates cell proliferation and migration in cancer cells.

Authors - Zeng Zih-Ming ZM
  • KD Validated
humanWB

J Agric Food Chem

Capsaicin-mediated tNOX (ENOX2) up-regulation enhances cell proliferation and migration in vitro and in vivo.

Authors - Liu Nei-Chi NC
humanWB

Biochem Biophys Res Commun

RNA interference targeting tNOX attenuates cell migration via a mechanism that involves membrane association of Rac.

Authors - Liu Shan-Chi SC
  • KD Validated
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