Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells, mouse ovary tissue, PC-3 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IP | See 3 publications below |
Product Information
10423-1-AP targets ENOX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ENOX2 fusion protein Ag0674 相同性解析による交差性が予測される生物種 |
| Full Name | ecto-NOX disulfide-thiol exchanger 2 |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC019254 |
| Gene Symbol | ENOX2 |
| Gene ID (NCBI) | 10495 |
| RRID | AB_2099483 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16206 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tumor-associated NADH oxidase (ENOX2) as a member of a family of growth-related NADH (or hydroquinone) oxidases. It functions as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ENOX2 antibody 10423-1-AP | Download protocol |
| IHC protocol for ENOX2 antibody 10423-1-AP | Download protocol |
| IF protocol for ENOX2 antibody 10423-1-AP | Download protocol |
| IP protocol for ENOX2 antibody 10423-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Med Chem Synthesis and characterization of 4,11-diaminoanthra[2,3-b]furan-5,10-diones: tumor cell apoptosis through tNOX-modulated NAD+/NADH ratio and SIRT1. | ||
Am J Cancer Res Capsaicin acts through tNOX (ENOX2) to induce autophagic apoptosis in p53-mutated HSC-3 cells but autophagy in p53-functional SAS oral cancer cells. | ||
Am J Cancer Res Capsaicin exerts therapeutic effects by targeting tNOX-SIRT1 axis and augmenting ROS-dependent autophagy in melanoma cancer cells. | ||
Exp Cell Res Phosphorylation of serine-504 of tNOX (ENOX2) modulates cell proliferation and migration in cancer cells.
| ||
J Agric Food Chem Capsaicin-mediated tNOX (ENOX2) up-regulation enhances cell proliferation and migration in vitro and in vivo. | ||
Biochem Biophys Res Commun RNA interference targeting tNOX attenuates cell migration via a mechanism that involves membrane association of Rac.
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