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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ENOX2 Polyclonal antibody

ENOX2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10423

Synonyms

Protein disulfide-thiol oxidoreductase, Hydroquinone [NADH] oxidase, Ecto-NOX disulfide-thiol exchanger 2, COVA1, APK1 antigen

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated ENOX2 Polyclonal ant (CL488-10423)

    IF Staining of MCF-7 using CL488-10423

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 ENOX2 antibody (CL488-10423) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated ENOX2 Polyclonal ant (CL488-10423)

    FC experiment of MCF-7 using CL488-10423

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated ENOX2 Polyclonal antibody (CL488-10423)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10423 targets ENOX2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENOX2 fusion protein Ag0674 相同性解析による交差性が予測される生物種
Full Name ecto-NOX disulfide-thiol exchanger 2
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC019254
Gene symbol ENOX2
Gene ID (NCBI) 10495
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tumor-associated NADH oxidase (ENOX2) as a member of a family of growth-related NADH (or hydroquinone) oxidases. It functions as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ENOX2 antibody CL488-10423Download protocol
FC protocol for CL Plus 488 ENOX2 antibody CL488-10423Download protocol
Standard Protocols
Click here to view our Standard Protocols