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ENPP2 Monoclonal antibody

ENPP2 Monoclonal Antibody for WB, ELISA
Cat No. 68724-1-Ig
Clone No.2A10D9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, ELISA

AUTOTAXIN, E-NPP 2, EC:3.1.4.39, E NPP 2, ATX X

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 1000 μg/ml
20 μL, 1000 μg/ml
150 μL, 1000 μg/ml

¥67,000/ 150 μL

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約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inpig brain tissue, HuH-7 cells, U-87 MG cells, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68724-1-Ig targets ENPP2 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityrat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ENPP2 fusion protein Ag5499 相同性解析による交差性が予測される生物種
Full Name ectonucleotide pyrophosphatase/phosphodiesterase 2
Calculated molecular weight 99 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC034961
Gene Symbol ENPP2
Gene ID (NCBI) 5168
RRIDAB_3670416
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13822
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. The calculated molecular weight of ENPP2 is about 100 kDa (PMID: 22301782), but modified ENPP2 is 120 kDa (PMID: 19808661). ENPP2 Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931).

Protocols

Product Specific Protocols
WB protocol for ENPP2 antibody 68724-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Pharm Pharmacol

Renoprotective effect of liraglutide on diabetic nephropathy by modulation of Krüppel-like transcription factor 5 expression in rats

Authors - Anfal F Bin Dayel
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