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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ENPP2 Monoclonal antibody, PBS Only

ENPP2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A10D9

Cat no : 68724-1-PBS

Synonyms

AUTOTAXIN, E-NPP 2, EC:3.1.4.39, E NPP 2, ATX X

Validation Data Gallery

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  • Western Blot (WB) analysis of HuH-7 cells using ENPP2 Monoclonal antibody (68724-1-Ig)

    WB analysis of HuH-7 using 68724-1-Ig (same clone as 68724-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-87 MG cells using ENPP2 Monoclonal antibody (68724-1-Ig)

    WB analysis of U-87 MG using 68724-1-Ig (same clone as 68724-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ENPP2 Monoclonal antibody (68724-1-Ig)

    WB analysis using 68724-1-Ig (same clone as 68724-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68724-1-PBS targets ENPP2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ENPP2 fusion protein Ag5499 相同性解析による交差性が予測される生物種
Full Name ectonucleotide pyrophosphatase/phosphodiesterase 2
Calculated molecular weight 99 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC034961
Gene symbol ENPP2
Gene ID (NCBI) 5168
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. The calculated molecular weight of ENPP2 is about 100 kDa (PMID: 22301782), but modified ENPP2 is 120 kDa (PMID: 19808661). ENPP2 Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931).