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Validation Data Gallery

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  • Western Blot (WB) analysis of HuH-7 cells using ENPP2 Monoclonal antibody (68724-1-Ig)

    WB analysis of HuH-7 using 68724-1-Ig (same clone as 68724-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-87 MG cells using ENPP2 Monoclonal antibody (68724-1-Ig)

    WB analysis of U-87 MG using 68724-1-Ig (same clone as 68724-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ENPP2 Monoclonal antibody (68724-1-Ig)

    WB analysis using 68724-1-Ig (same clone as 68724-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

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ENPP2 Monoclonal antibody, PBS Only

ENPP2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68724-1-PBS
Clone No.2A10D9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, Indirect ELISA

Synonyms

AUTOTAXIN, E-NPP 2, EC:3.1.4.39, E NPP 2, ATX X

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68724-1-PBS targets ENPP2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ENPP2 fusion protein Ag5499 相同性解析による交差性が予測される生物種
Full Name ectonucleotide pyrophosphatase/phosphodiesterase 2
Calculated molecular weight 99 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC034961
Gene Symbol ENPP2
Gene ID (NCBI) 5168
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13822
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. The calculated molecular weight of ENPP2 is about 100 kDa (PMID: 22301782), but modified ENPP2 is 120 kDa (PMID: 19808661). ENPP2 Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931).