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EPCAM/CD326 Monoclonal antibody
EPCAM/CD326 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2A2D5
Cat no : 66316-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HaCaT cells, A431 cells, T-47D cells, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue, human stomach cancer tissue |
| Positive IF/ICC detected in | MCF-7 cells, HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| FC | See 1 publications below |
Product Information
66316-1-Ig targets EPCAM/CD326 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | EPCAM/CD326 fusion protein Ag15393 相同性解析による交差性が予測される生物種 |
| Full Name | epithelial cell adhesion molecule |
| Calculated molecular weight | 314 aa, 35 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC014785 |
| Gene symbol | EPCAM |
| Gene ID (NCBI) | 4072 |
| RRID | AB_2881697 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EPCAM/CD326 antibody 66316-1-Ig | Download protocol |
| IHC protocol for EPCAM/CD326 antibody 66316-1-Ig | Download protocol |
| IF protocol for EPCAM/CD326 antibody 66316-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Biomater Tumor cell membrane-based peptide delivery system targeting the tumor microenvironment for cancer immunotherapy and diagnosis. | ||
Biosens Bioelectron Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes. | ||
Mol Ther Nucleic Acids miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance. | ||
Cell Death Dis Mutations in the coat complex II component SEC23B promote colorectal cancer metastasis. | ||
Front Pharmacol Photo-functionalized TiO2 film for facile immobilization of EpCAM antibodies and efficient enrichment of circulating tumor cells |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Guorong (Verified Customer) (03-31-2022) | Seems not good for ARPE-19 cells
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