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  • Featured Product
  • KD/KO Validated

EPCAM/CD326 Monoclonal antibody, PBS Only

EPCAM/CD326 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A2D5

Cat no : 66316-1-PBS

Synonyms

EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPCAM/CD326 Monoclonal antibody (66316-1-Ig)

    WB analysis using 66316-1-Ig (same clone as 66316-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using EPCAM/CD326 Monoclonal antibody (66316-1-Ig)

    WB analysis of A431 using 66316-1-Ig (same clone as 66316-1-PBS)

    WB result of EPCAM antibody (66316-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using EPCAM/CD326 Monoclonal antibody (66316-1-Ig)

    WB analysis of T-47D using 66316-1-Ig (same clone as 66316-1-PBS)

    T-47D, MCF-7 and A431 cells were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EPCAM/CD326 Monoclonal antibody (66316-1-Ig)

    IHC staining of human breast cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66316-1-Ig (EPCAM Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human stomach cancer tissue using EPCAM/CD326 Monoclonal antibody (66316-1-Ig)

    IF Staining of human stomach cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using EPCAM/CD326 Monoclonal antibody (66316-1-Ig)

    IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using EPCAM/CD326 Monoclonal antibody (66316-1-Ig)

    IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using EPCAM/CD326 Monoclonal antibody (66316-1-Ig)

    IF Staining of MCF-7 using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EPCAM antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using EPCAM/CD326 Monoclonal antibody (66316-1-Ig)

    IF Staining of HT-29 using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66316-1-PBS targets EPCAM/CD326 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EPCAM/CD326 fusion protein Ag15393 相同性解析による交差性が予測される生物種
Full Name epithelial cell adhesion molecule
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC014785
Gene symbol EPCAM
Gene ID (NCBI) 4072
RRIDAB_2881697
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)