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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclonal antibody

EPCAM/CD326 Polyclonal Antibody for IF/ICC, FC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21050

Synonyms

EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclon (CL488-21050)

    IF Staining of MCF-7 using CL488-21050

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 EPCAM/CD326 antibody (CL488-21050) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclon (CL488-21050)

    FC experiment of MCF-7 using CL488-21050

    1x10^6 MCF-7 cells were surface stained with 0.8 ug CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclonal antibody (CL488-21050) (red), or 0.8 ug Isotype Control (blue). Cells were not fixed.

  • Flow cytometry (FC) experiment of HT-29 cells using CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclon (CL488-21050)

    FC experiment of HT-29 using CL488-21050

    1x10^6 HT-29 cells were surface stained with 0.8 ug CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclonal antibody (CL488-21050) (red), or 0.8 ug Control Antibody (blue). Cells were not fixed.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclon (CL488-21050)

    FC experiment of A431 using CL488-21050

    1x10^6 A431 cells were surface stained with 0.4 ug CoraLite® Plus 488-conjugated EPCAM/CD326 Polyclonal antibody (CL488-21050) (red), or 0.4 ug Control Antibody (blue). Cells were not fixed.

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC detected inMCF-7 cells, A431 cells, HT-29 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21050 targets EPCAM/CD326 in IF/ICC, FC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPCAM/CD326 fusion protein Ag15393 相同性解析による交差性が予測される生物種
Full Name epithelial cell adhesion molecule
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC014785
Gene symbol EPCAM
Gene ID (NCBI) 4072
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EPCAM/CD326 antibody CL488-21050Download protocol
FC protocol for CL Plus 488 EPCAM/CD326 antibody CL488-21050Download protocol
Standard Protocols
Click here to view our Standard Protocols