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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated EPCAM/CD326 Monoclonal ant (CL594-66316)

    IF Staining of MCF-7 using CL594-66316

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 EPCAM/CD326 antibody (CL594-66316, Clone: 2A2D5 ) at dilution of 1:200.

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  • KD/KO Validated

CoraLite®594-conjugated EPCAM/CD326 Monoclonal antibody

EPCAM/CD326 Monoclonal Antibody for IF/ICC
Cat No. CL594-66316
Clone No.2A2D5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Synonyms

EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66316 targets EPCAM/CD326 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EPCAM/CD326 fusion protein Ag15393 相同性解析による交差性が予測される生物種
Full Name epithelial cell adhesion molecule
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC014785
Gene Symbol EPCAM
Gene ID (NCBI) 4072
RRIDAB_2883555
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16422
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Protocols

Product Specific Protocols
IF protocol for CL594 EPCAM/CD326 antibody CL594-66316Download protocol
Standard Protocols
Click here to view our Standard Protocols