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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPHB2 Recombinant antibody (83277-1-RR)

    WB analysis using 83277-1-RR (same clone as 83277-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83277-1-RR (EPHB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83277-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using EPHB2 Recombinant antibody (83277-1-RR)

    FC experiment of HepG2 using 83277-1-RR (same clone as 83277-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug EPHB2 Recombinant antibody (83277-1-RR, Clone:230111A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83277-1-PBS in a different storage buffer formulation.

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EPHB2 Recombinant antibody, PBS Only

EPHB2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 83277-1-PBS
Clone No.230111A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EK5, EC:2.7.10.1, DRT, Developmentally-regulated Eph-related tyrosine kinase, CAPB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83277-1-PBS targets EPHB2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPHB2 fusion protein Ag28786 相同性解析による交差性が予測される生物種
Full Name EPH receptor B2
Calculated molecular weight 987 aa, 108 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC018763
Gene Symbol EPHB2
Gene ID (NCBI) 2048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP29323
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.