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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EPHB2 Recombinant antibody

EPHB2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230111A5

Cat no : 83277-1-RR

Synonyms

EK5, EC:2.7.10.1, DRT, Developmentally-regulated Eph-related tyrosine kinase, CAPB

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EPHB2 Recombinant antibody (83277-1-RR)

    WB analysis using 83277-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83277-1-RR (EPHB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using EPHB2 Recombinant antibody (83277-1-RR)

    FC experiment of HepG2 using 83277-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug EPHB2 Recombinant antibody (83277-1-RR, Clone:230111A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHepG2 cells, U-251 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 83277-1-RR is EPHB2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPHB2 fusion protein Ag28786 相同性解析による交差性が予測される生物種
Full Name EPH receptor B2
Calculated molecular weight 987 aa, 108 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC018763
Gene symbol EPHB2
Gene ID (NCBI) 2048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EPHB2 antibody 83277-1-RRDownload protocol
FC protocol for EPHB2 antibody 83277-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

tsRNA-GlyGCC promotes colorectal cancer progression and 5-FU resistance by regulating SPIB

Authors - Rong Xu
View Article