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EPHX2 Monoclonal antibody
EPHX2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
4H6C8
Cat no : 67322-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, A549 cells, Jurkat cells, pig liver tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67322-1-Ig targets EPHX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
Tested Reactivity | human, pig |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | EPHX2 fusion protein Ag4870 相同性解析による交差性が予測される生物種 |
Full Name | epoxide hydrolase 2, cytoplasmic |
Calculated molecular weight | 63 kDa |
Observed molecular weight | 56-63 kDa |
GenBank accession number | BC013874 |
Gene symbol | EPHX2 |
Gene ID (NCBI) | 2053 |
RRID | AB_2882582 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).
Protocols
Product Specific Protocols | |
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WB protocol for EPHX2 antibody 67322-1-Ig | Download protocol |
IHC protocol for EPHX2 antibody 67322-1-Ig | Download protocol |
IF protocol for EPHX2 antibody 67322-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |