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EPHX2 Monoclonal antibody

EPHX2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4H6C8

Cat no : 67322-1-Ig

Synonyms

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPHX2 Monoclonal antibody (67322-1-Ig)

    WB analysis using 67322-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67322-1-Ig (EPHX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using EPHX2 Monoclonal antibody (67322-1-Ig)

    WB analysis of A549 using 67322-1-Ig

    WB result of EPHX2 antibody (67322-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX2 transfected A549 cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EPHX2 Monoclonal antibody (67322-1-Ig)

    IHC staining of human breast cancer using 67322-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EPHX2 Monoclonal antibody (67322-1-Ig)

    IHC staining of human breast cancer using 67322-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using EPHX2 Monoclonal antibody (67322-1-Ig)

    IF Staining of A549 using 67322-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using EPHX2 antibody (67322-1-Ig, Clone: 4H6C8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inLNCaP cells, A549 cells, Jurkat cells, pig liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67322-1-Ig targets EPHX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EPHX2 fusion protein Ag4870 相同性解析による交差性が予測される生物種
Full Name epoxide hydrolase 2, cytoplasmic
Calculated molecular weight 63 kDa
Observed molecular weight 56-63 kDa
GenBank accession numberBC013874
Gene symbol EPHX2
Gene ID (NCBI) 2053
RRIDAB_2882582
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

Protocols

Product Specific Protocols
WB protocol for EPHX2 antibody 67322-1-IgDownload protocol
IHC protocol for EPHX2 antibody 67322-1-IgDownload protocol
IF protocol for EPHX2 antibody 67322-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols