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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FITC Plus-conjugated EPHX2 Monoclonal antibody (FITC-67322)

    IF Staining of A549 using FITC-67322

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FITC Plus EPHX2 antibody (FITC-67322, Clone: 4H6C8 ) at dilution of 1:50.

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  • Featured Product
  • KD/KO Validated

FITC Plus-conjugated EPHX2 Monoclonal antibody

EPHX2 Monoclonal Antibody for IF/ICC
Cat No. FITC-67322
Clone No.4H6C8

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

IF/ICC

Synonyms

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  FITC Plus
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-67322 targets EPHX2 in IF/ICC applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EPHX2 fusion protein Ag4870 相同性解析による交差性が予測される生物種
Full Name epoxide hydrolase 2, cytoplasmic
Calculated molecular weight 63 kDa
Observed molecular weight 56-63 kDa
GenBank accession numberBC013874
Gene Symbol EPHX2
Gene ID (NCBI) 2053
RRIDAB_3673875
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP34913
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

Protocols

Product Specific Protocols
IF protocol for FITC Plus EPHX2 antibody FITC-67322Download protocol
Standard Protocols
Click here to view our Standard Protocols