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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using Biotin-conjugated EPHX2 Polyclonal antibody (Biotin-10833)

    IHC staining of mouse brain using Biotin-10833

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-10833 (EPHX2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Biotin-conjugated EPHX2 Polyclonal antibody

EPHX2 Polyclonal Antibody for IHC
Cat No. Biotin-10833

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-10833 targets EPHX2 in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPHX2 fusion protein Ag1283 相同性解析による交差性が予測される生物種
Full Name epoxide hydrolase 2, cytoplasmic
Calculated molecular weight 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC013874
Gene Symbol EPHX2
Gene ID (NCBI) 2053
RRIDAB_3672368
Conjugate Biotin
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP34913
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

Protocols

Product Specific Protocols
IHC protocol for Biotin EPHX2 antibody Biotin-10833Download protocol
Standard Protocols
Click here to view our Standard Protocols