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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EPRS Monoclonal antibody, PBS Only

EPRS Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7G2

Cat no : 67712-1-PBS

Synonyms

DKFZp313B047, EARS, EPRS, GLNS, GLUPRORS, GluRS Prolyl tRNA synthetase, Glutamate tRNA ligase, PARS, PIG32, Proline tRNA ligase, QARS, QPRS

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis using 67712-1-Ig (same clone as 67712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis of HSC-T6 using 67712-1-Ig (same clone as 67712-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis of PC-12 using 67712-1-Ig (same clone as 67712-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis of NIH/3T3 using 67712-1-Ig (same clone as 67712-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using EPRS Monoclonal antibody (67712-1-Ig)

    IP experiment of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    IP result of anti-EPRS (IP:67712-1-Ig, 4ug; Detection:67712-1-Ig 1:10000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPRS Monoclonal antibody (67712-1-Ig)

    IHC staining of human lung cancer using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67712-1-Ig (EPRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPRS Monoclonal antibody (67712-1-Ig)

    IHC staining of human lung cancer using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67712-1-Ig (EPRS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPRS Monoclonal antibody (67712-1-Ig)

    IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPRS Monoclonal antibody (67712-1-Ig)

    IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67712-1-PBS targets EPRS in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EPRS fusion protein Ag19184 相同性解析による交差性が予測される生物種
Full Name glutamyl-prolyl-tRNA synthetase
Calculated molecular weight 1512 aa, 171 kDa
Observed molecular weight171 kDa
GenBank accession numberBC126275
Gene symbol EPRS
Gene ID (NCBI) 2058
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)