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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis using 67712-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis of HSC-T6 using 67712-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis of PC-12 using 67712-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis of NIH/3T3 using 67712-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using EPRS Monoclonal antibody (67712-1-Ig)

    IP experiment of HeLa using 67712-1-Ig

    IP result of anti-EPRS (IP:67712-1-Ig, 4ug; Detection:67712-1-Ig 1:10000) with HeLa cells lysate 1085 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPRS Monoclonal antibody (67712-1-Ig)

    IHC staining of human lung cancer using 67712-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67712-1-Ig (EPRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPRS Monoclonal antibody (67712-1-Ig)

    IHC staining of human lung cancer using 67712-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67712-1-Ig (EPRS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPRS Monoclonal antibody (67712-1-Ig)

    IF Staining of HeLa using 67712-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPRS Monoclonal antibody (67712-1-Ig)

    IF Staining of HeLa using 67712-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of BV-2 cells using EPRS Monoclonal antibody (67712-1-Ig)

    IF Staining of BV-2 using 67712-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed BV-2 cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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EPRS Monoclonal antibody

EPRS Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 67712-1-Ig
Clone No.1B7G2
Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EPRS1, EARS, Cell proliferation-inducing gene 32 protein, Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, HeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, BV-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67712-1-Ig targets EPRS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EPRS fusion protein Ag19184 相同性解析による交差性が予測される生物種
Full Name glutamyl-prolyl-tRNA synthetase
Calculated molecular weight 1512 aa, 171 kDa
Observed molecular weight171 kDa
GenBank accession numberBC126275
Gene Symbol EPRS
Gene ID (NCBI) 2058
RRIDAB_2882902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07814
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)

Protocols

Product Specific Protocols
WB protocol for EPRS antibody 67712-1-IgDownload protocol
IHC protocol for EPRS antibody 67712-1-IgDownload protocol
IF protocol for EPRS antibody 67712-1-IgDownload protocol
IP protocol for EPRS antibody 67712-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,IHC

Kaohsiung J Med Sci

Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells

Authors - Li-Qi Lin
View Article