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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated EPRS Monoclonal antibody

EPRS Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1B7G2

Cat no : CL647-67712

Synonyms

DKFZp313B047, EARS, EPRS, GLNS, GLUPRORS, GluRS Prolyl tRNA synthetase, Glutamate tRNA ligase, PARS, PIG32, Proline tRNA ligase, QARS, QPRS

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 647-conjugated EPRS Monoclonal anti (CL647-67712)

    IF Staining of HeLa using CL647-67712

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 647 EPRS antibody (CL647-67712, Clone: 1B7G2 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67712 targets EPRS in IF/ICC applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EPRS fusion protein Ag19184 相同性解析による交差性が予測される生物種
Full Name glutamyl-prolyl-tRNA synthetase
Calculated molecular weight 1512 aa, 171 kDa
GenBank accession numberBC126275
Gene symbol EPRS
Gene ID (NCBI) 2058
RRIDAB_2920300
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 EPRS antibody CL647-67712Download protocol
Standard Protocols
Click here to view our Standard Protocols