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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using ERAP2 Monoclonal antibody (67477-1-Ig)

    WB analysis of A549 using 67477-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ERAP2 Monoclonal antibody (67477-1-Ig)

    WB analysis using 67477-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ERAP2 Monoclonal antibody (67477-1-Ig)

    WB analysis of HepG2 using 67477-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using ERAP2 Monoclonal antibody (67477-1-Ig)

    WB analysis of HSC-T6 using 67477-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ERAP2 Monoclonal antibody (67477-1-Ig)

    WB analysis of HeLa using 67477-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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ERAP2 Monoclonal antibody

ERAP2 Monoclonal Antibody for WB, ELISA
Cat No. 67477-1-Ig
Clone No.2B11A2
Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat and More (1)

Applications

WB, ELISA

Synonyms

ERAP2, FLJ23633, FLJ23701, FLJ23807, L RAP, LRAP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67477-1-Ig targets ERAP2 in WB, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ERAP2 fusion protein Ag30061 相同性解析による交差性が予測される生物種
Full Name endoplasmic reticulum aminopeptidase 2
Calculated molecular weight 110 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC065240
Gene Symbol ERAP2
Gene ID (NCBI) 64167
RRIDAB_2882704
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P179
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ERAP2(Endoplasmic reticulum aminopeptidase 2) is also named as LRAP(Leukocyte-derived arginine aminopeptidase). It plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. ERAP2 can form heterodimers with ERAP1(PMID:15908954). Defects in the expression of ERAP2 may cause improper antigen processing, possibly leading to favor tumor escape from the immune surveillance. ERAP2 has the calculated molecular mass of 105-110 kDa, and apparent molecular mass of 120 kDa duo to the glycosylation.

Protocols

Product Specific Protocols
WB protocol for ERAP2 antibody 67477-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

ERAP2 as a potential biomarker for predicting gemcitabine response in patients with pancreatic cancer

Authors - Pian Yu
View Article