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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERAP2 Monoclonal antibody, PBS Only

ERAP2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B11A2

Cat no : 67477-1-PBS

Synonyms

ERAP2, FLJ23633, FLJ23701, FLJ23807, L RAP, LRAP

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using ERAP2 Monoclonal antibody (67477-1-Ig)

    WB analysis of A549 using 67477-1-Ig (same clone as 67477-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67477-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ERAP2 Monoclonal antibody (67477-1-Ig)

    WB analysis using 67477-1-Ig (same clone as 67477-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67477-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ERAP2 Monoclonal antibody (67477-1-Ig)

    WB analysis of HepG2 using 67477-1-Ig (same clone as 67477-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67477-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ERAP2 Monoclonal antibody (67477-1-Ig)

    WB analysis of HSC-T6 using 67477-1-Ig (same clone as 67477-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67477-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ERAP2 Monoclonal antibody (67477-1-Ig)

    WB analysis of HeLa using 67477-1-Ig (same clone as 67477-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67477-1-Ig (ERAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67477-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67477-1-PBS targets ERAP2 in WB, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ERAP2 fusion protein Ag30061 相同性解析による交差性が予測される生物種
Full Name endoplasmic reticulum aminopeptidase 2
Calculated molecular weight 110 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC065240
Gene symbol ERAP2
Gene ID (NCBI) 64167
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ERAP2(Endoplasmic reticulum aminopeptidase 2) is also named as LRAP(Leukocyte-derived arginine aminopeptidase). It plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. ERAP2 can form heterodimers with ERAP1(PMID:15908954). Defects in the expression of ERAP2 may cause improper antigen processing, possibly leading to favor tumor escape from the immune surveillance. ERAP2 has the calculated molecular mass of 105-110 kDa, and apparent molecular mass of 120 kDa duo to the glycosylation.