ERCC1 Monoclonal antibody
ERCC1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1H4D5
Cat no : 66275-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
66275-1-Ig targets ERCC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ERCC1 fusion protein Ag6417 相同性解析による交差性が予測される生物種 |
| Full Name | excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC052813 |
| Gene symbol | ERCC1 |
| Gene ID (NCBI) | 2067 |
| RRID | AB_2881658 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERCC1 antibody 66275-1-Ig | Download protocol |
| IF protocol for ERCC1 antibody 66275-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
