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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using ERCC1 Monoclonal antibody (66275-1-Ig)

    WB analysis of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66275-1-Ig (ERCC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66275-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ERCC1 Monoclonal antibody (66275-1-Ig)

    IF Staining of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed A431 cells using 66275-1-Ig(ERCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66275-1-PBS in a different storage buffer formulation.

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ERCC1 Monoclonal antibody, PBS Only

ERCC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66275-1-PBS
Clone No.1H4D5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

COFS4, ERCC1, UV20

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66275-1-PBS targets ERCC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERCC1 fusion protein Ag6417 相同性解析による交差性が予測される生物種
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Calculated molecular weight 33 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC052813
Gene Symbol ERCC1
Gene ID (NCBI) 2067
RRIDAB_2881658
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07992
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005)