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KD/KO Validated

ERG Polyclonal antibody

Name: ERG Polyclonal antibody
Brand: Proteintech
SKU: 14356-1-AP

ERG Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14356-1-AP

Synonyms

Transforming protein ERG, Transcriptional regulator ERG, erg 3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of MCF-7 using 14356-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14356-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14356-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14356-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 14356-1-AP
IP result of anti-ERG (IP:14356-1-AP, 4ug; Detection:14356-1-AP 1:500) with MCF-7 cells lysate 800ug.
IHC staining of human prostate cancer using 14356-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 14356-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14356-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14356-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 14356-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 14356-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 14356-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14356-1-AP (ERG antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of MCF-7 using 14356-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human ERG (14356-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 14356-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF-7 cells, HEK-293 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human prostate cancer tissue, mouse brain tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:600-1:2400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

14356-1-AP targets ERG in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ERG fusion protein Ag5708 相同性解析による交差性が予測される生物種
Full Name	v-ets erythroblastosis virus E26 oncogene homolog (avian)
Calculated molecular weight	55 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC040168
Gene symbol	ERG
Gene ID (NCBI)	2078
RRID	AB_2098423
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ERG (ETS-related gene) is a member of the ETS family of transcription factors, which are downstream effectors of mitogenic signaling transduction pathway. ERG is a key regulator of cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. ERG is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia.

Protocols

Product Specific Protocols
WB protocol for ERG antibody 14356-1-AP	Download protocol
IHC protocol for ERG antibody 14356-1-AP	Download protocol
IF protocol for ERG antibody 14356-1-AP	Download protocol
IP protocol for ERG antibody 14356-1-AP	Download protocol
FC protocol for ERG antibody 14356-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	iScience Resident vascular Sca1+ progenitors differentiate into endothelial cells in vascular remodeling via miR-145-5p/ERG signaling pathway Authors - Zhangquan Ying View Article
human	WB	Cell Biosci Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer Authors - Qiong Wang View Article
human	WB	J Cancer miR-223-5p targeting ERG inhibits prostate cancer cell proliferation and migration. Authors - Yongbao Wei KD Validated View Article
human,mouse	WB,IF	Cell Death Discov HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells. Authors - Feng Yao View Article
human	IHC	Exp Ther Med Rare angiolymphoid hyperplasia with eosinophilia examined through fine needle aspiration cytology, histopathology and immunophenotypic characterization: A case report Authors - Xiaohua Wang View Article
human	WB	Nat Commun DNA framework-engineered chimeras platform enables selectively targeted protein degradation Authors - Li Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERG Polyclonal antibody

ERG Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of MCF-7 using 14356-1-AP

WB analysis of HEK-293 using 14356-1-AP

WB analysis of MCF-7 using 14356-1-AP

WB analysis of MCF-7 using 14356-1-AP

IP experiment of MCF-7 using 14356-1-AP

IHC staining of human prostate cancer using 14356-1-AP

IHC staining of human prostate cancer using 14356-1-AP

IHC staining of mouse brain using 14356-1-AP

IHC staining of mouse brain using 14356-1-AP

IHC staining of human tonsillitis using 14356-1-AP

IHC staining of human tonsillitis using 14356-1-AP

IF Staining of MCF-7 using 14356-1-AP

FC experiment of MCF-7 using 14356-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Resident vascular Sca1+ progenitors differentiate into endothelial cells in vascular remodeling via miR-145-5p/ERG signaling pathway

Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer

miR-223-5p targeting ERG inhibits prostate cancer cell proliferation and migration.

HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells.

Rare angiolymphoid hyperplasia with eosinophilia examined through fine needle aspiration cytology, histopathology and immunophenotypic characterization: A case report

DNA framework-engineered chimeras platform enables selectively targeted protein degradation

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