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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ERG Polyclonal antibody

ERG Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14356

Synonyms

Transforming protein ERG, Transcriptional regulator ERG, erg 3

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated ERG Polyclonal antib (CL488-14356)

    FC experiment of MCF-7 using CL488-14356

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated ERG Polyclonal antibody (CL488-14356)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14356 targets ERG in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERG fusion protein Ag5708 相同性解析による交差性が予測される生物種
Full Name v-ets erythroblastosis virus E26 oncogene homolog (avian)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC040168
Gene symbol ERG
Gene ID (NCBI) 2078
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERG (ETS-related gene) is a member of the ETS family of transcription factors, which are downstream effectors of mitogenic signaling transduction pathway. ERG is a key regulator of cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. ERG is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 ERG antibody CL488-14356Download protocol
Standard Protocols
Click here to view our Standard Protocols