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CoraLite® Plus 488-conjugated ERK1/2 Polyclonal antibody
ERK1/2 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-11257
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-11257 targets ERK1/2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ERK1/2 fusion protein Ag1759 相同性解析による交差性が予測される生物種 |
Full Name | mitogen-activated protein kinase 3 |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 38-44 kDa |
GenBank accession number | BC013992 |
Gene symbol | ERK1 |
Gene ID (NCBI) | 5595 |
RRID | AB_2919021 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 ERK1/2 antibody CL488-11257 | Download protocol |
FC protocol for CL Plus 488 ERK1/2 antibody CL488-11257 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |