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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERN2 Monoclonal antibody

ERN2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G6A10

Cat no : 67289-1-Ig

Synonyms

ERN2, hIRE2p, Inositol requiring protein 2, Ire1 beta, IRE1b, IRE2

Validation Data Gallery

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  • Western Blot (WB) analysis of JAR cells using ERN2 Monoclonal antibody (67289-1-Ig)

    WB analysis of JAR using 67289-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67289-1-Ig (ERN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ERN2 Monoclonal antibody (67289-1-Ig)

    WB analysis of A549 using 67289-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67289-1-Ig (ERN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERN2 Monoclonal antibody (67289-1-Ig)

    IHC staining of human liver cancer using 67289-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67289-1-Ig (ERN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERN2 Monoclonal antibody (67289-1-Ig)

    IHC staining of human liver cancer using 67289-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67289-1-Ig (ERN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJAR cells, A549 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67289-1-Ig targets ERN2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ERN2 fusion protein Ag29452 相同性解析による交差性が予測される生物種
Full Name endoplasmic reticulum to nucleus signaling 2
Observed molecular weight 102 kDa
GenBank accession numberBC157113
Gene symbol ERN2
Gene ID (NCBI) 10595
RRIDAB_2882555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ERN2 antibody 67289-1-IgDownload protocol
IHC protocol for ERN2 antibody 67289-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols