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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERN2 Monoclonal antibody, PBS Only

ERN2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G6A10

Cat no : 67289-1-PBS

Synonyms

ERN2, hIRE2p, Inositol requiring protein 2, Ire1 beta, IRE1b, IRE2

Validation Data Gallery

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  • Western Blot (WB) analysis of JAR cells using ERN2 Monoclonal antibody (67289-1-Ig)

    WB analysis of JAR using 67289-1-Ig (same clone as 67289-1-PBS)

    JAR cells were subjected to SDS PAGE followed by western blot with 67289-1-Ig (ERN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67289-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using ERN2 Monoclonal antibody (67289-1-Ig)

    WB analysis of A549 using 67289-1-Ig (same clone as 67289-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67289-1-Ig (ERN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67289-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERN2 Monoclonal antibody (67289-1-Ig)

    IHC staining of human liver cancer using 67289-1-Ig (same clone as 67289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67289-1-Ig (ERN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67289-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERN2 Monoclonal antibody (67289-1-Ig)

    IHC staining of human liver cancer using 67289-1-Ig (same clone as 67289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67289-1-Ig (ERN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67289-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67289-1-PBS targets ERN2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ERN2 fusion protein Ag29452 相同性解析による交差性が予測される生物種
Full Name endoplasmic reticulum to nucleus signaling 2
Observed molecular weight 102 kDa
GenBank accession numberBC157113
Gene symbol ERN2
Gene ID (NCBI) 10595
RRIDAB_2882555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.