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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERP29 Monoclonal antibody

ERP29 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1H8B2

Cat no : 67675-1-Ig

Synonyms

C12orf8, ERp28, ERP29, ERp31, PDI DB, PDIA9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERP29 Monoclonal antibody (67675-1-Ig)

    WB analysis using 67675-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67675-1-Ig (ERP29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IHC staining of human liver using 67675-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IHC staining of human liver using 67675-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IHC staining of human kidney using 67675-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IHC staining of human kidney using 67675-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IF Staining of human kidney using 67675-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ERP29 antibody (67675-1-Ig, Clone: 1H8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IF Staining of human kidney using 67675-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ERP29 antibody (67675-1-Ig, Clone: 1H8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67675-1-Ig targets ERP29 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ERP29 fusion protein Ag30262 相同性解析による交差性が予測される生物種
Full Name endoplasmic reticulum protein 29
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC101495
Gene symbol ERP29
Gene ID (NCBI) 10961
RRIDAB_2882870
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ERP29 antibody 67675-1-IgDownload protocol
IHC protocol for ERP29 antibody 67675-1-IgDownload protocol
IF protocol for ERP29 antibody 67675-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Cell Biol

Competition for calnexin binding regulates secretion and turnover of misfolded GPI-anchored proteins

Authors - Amber M Cheatham
View Article