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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERP29 Monoclonal antibody (67675-1-Ig)

    WB analysis using 67675-1-Ig (same clone as 67675-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67675-1-Ig (ERP29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IHC staining of human liver using 67675-1-Ig (same clone as 67675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IHC staining of human liver using 67675-1-Ig (same clone as 67675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IHC staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IHC staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IF Staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ERP29 antibody (67675-1-Ig, Clone: 1H8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using ERP29 Monoclonal antibody (67675-1-Ig)

    IF Staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ERP29 antibody (67675-1-Ig, Clone: 1H8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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ERP29 Monoclonal antibody, PBS Only

ERP29 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67675-1-PBS
Clone No.1H8B2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

C12orf8, ERp28, ERP29, ERp31, PDI DB, PDIA9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67675-1-PBS targets ERP29 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ERP29 fusion protein Ag30262 相同性解析による交差性が予測される生物種
Full Name endoplasmic reticulum protein 29
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC101495
Gene Symbol ERP29
Gene ID (NCBI) 10961
RRIDAB_2882870
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30040
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).