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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ESD Monoclonal antibody (67069-2-Ig)

    WB analysis using 67069-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67069-2-Ig (ESD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ESD Monoclonal antibody (67069-2-Ig)

    IF Staining of HEK-293 using 67069-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ESD antibody (67069-2-Ig, Clone: 2F5E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ESD Monoclonal antibody

ESD Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67069-2-Ig
Clone No.2F5E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12, EC:3.1.1.56

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, Caco-2 cells, HepG2 cells, Jurkat cells, K-562 cells, Ramos cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67069-2-Ig targets ESD in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ESD fusion protein Ag7487 相同性解析による交差性が予測される生物種
Full Name esterase D/formylglutathione hydrolase
Calculated molecular weight 31 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC001169
Gene Symbol ESD
Gene ID (NCBI) 2098
RRIDAB_3670378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.

Protocols

Product Specific Protocols
WB protocol for ESD antibody 67069-2-IgDownload protocol
IF protocol for ESD antibody 67069-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols