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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ESD Monoclonal antibody (67069-2-Ig)

    WB analysis using 67069-2-Ig (same clone as 67069-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67069-2-Ig (ESD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67069-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ESD Monoclonal antibody (67069-2-Ig)

    IF Staining of HEK-293 using 67069-2-Ig (same clone as 67069-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ESD antibody (67069-2-Ig, Clone: 2F5E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67069-2-PBS in a different storage buffer formulation.

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ESD Monoclonal antibody, PBS Only

ESD Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67069-2-PBS
Clone No.2F5E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12, EC:3.1.1.56

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67069-2-PBS targets ESD in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ESD fusion protein Ag7487 相同性解析による交差性が予測される生物種
Full Name esterase D/formylglutathione hydrolase
Calculated molecular weight 31 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC001169
Gene Symbol ESD
Gene ID (NCBI) 2098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10768
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.